Recording of a fluorescence spectrum with a spectrometer

VC3.3.1.3
Recording of a fluorescence spectrum with a spectrometer
Recording of a fluorescence spectrum with a spectrometer

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Description

In experiment C3.3.1.3, a solution of the fluorescent dye fluorescein is exposed to the light from a lamp. For this purpose, the spectrometer is arranged at a right angle to the lamp. In this way, the fluorescence spectrum of the dye can be recorded. This can be compared with the absorption spectrum from experiment C3.3.1.2.

Components of equipment sets

2 664470 Rectangular cuvette cell, glass, 10 x 10 mm LD
1 467252 Compact spectrometer, complete LD
1 458100  4  Halogen spotlight, 12 V/20 W LD
1 562791  4  Plug-in power supply, 12 V AC LD
1 30002  4  Stand base, V-shaped, small LD
1 30126  4  Stand rod 25 cm, 10 mm Ø LD
1 30111  4  Clamp with jaw clamp LD
1 6045672  8  Double microspatula, steel, 150 mm LD
1 602022  4  Beaker Boro 3.3, 100 ml, squat LD
1 664130  4  Beaker, Boro 3.3, 250 ml, squat LD
1 665754  4  Measuring cylinder 100 ml, with plastic base LD
1 665996  4  Graduated pipette, 5 ml LD
1 666003  4  Pipetting ball (Peleus ball) LD
1 665953  8  Dropping pipette, 7 x 150 mm, 10 pcs. LD
1 665954  4  Rubber bulbs, 10 pcs LD
1 30942  4  Colouring, red, 10 g LD
1 6720110  4  Fluoresceine, 25 g LD
1 6719740  4  Ethanol, denaturated, 250 ml LD
GHS02GHS07 H225 H319
1 6753400  4  Water, pure, 1 l LD
1 additionally required: PC with Windows 7 or higher with WIFI or USB connection

Experiment Instructions

PDF (Experiment description) PDF (Experiment description) C3.3.1.3 Recording of a fluorescence spectrum with a spectrometer
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