Separation of leaf pigments by thin layer chromatography

LB2.2.3.6
Separation of leaf pigments by thin layer chromatography
Separation of leaf pigments by thin layer chromatography

Description

In this experiment, leaf pigments are separated by using thin-layer chromatography (DC). For this, the leaf pigments are extracted from grass and then separated from each other with the help of silica gel foil and consequently made visible. The students realise that there are several different leaf dyes in one plant. This chromatography method is much more accurate than paper chromatography.

Components of equipment sets

1 6742220  4 
Petroleum ether, 40...70 °C, 1 l LD
GHS02GHS08GHS07GHS09 Danger H225 H304 H315 H336 H411 EUH066
1 6700400  4 
Acetone, 250 ml LD
GHS02GHS07 Danger H225 H319 EUH066 H336
1 6719500  4 
Acetic acid, 99%-100%, 250 ml LD
GHS02GHS05 Danger H226 H314 H290
1 6045682  4 
Powder spatula, steel, 185 mm LD
1 664154  4 
Watch glass dish 80 mm Ø LD
1 30945  4 
Universal pencil LD
1 667017  4 
Scissors 125 mm, round-ended LD
1 6659531  4 
Dropping pipette 150 mm x 7 mm Ø LD
1 6659541  8 
Rubber bulb LD
1 661035  8 
Round filter, Type 595, 125 mm Ø, Set of 100 LD
1 664182  4 
Petri dish, 100 x 15 mm, glass LD
2 602022  4 
Beaker Boro 3.3, 100 ml, squat LD
1 667092  4 
Mortar 30 ml, 56 mm x 36 mm LD
1 667091  4 
Pestle 88 mm LD
1 665009  4 
Funnel PP 75 mm Ø LD
1 610010  8 
Laboratory safety goggles LD
1 661062  4 
** Ready-to-use foil: Silica gel, 200 x 200 mm, set of 25 LD
1 665569  4 
Microcapillaries LD
1 6700400  4 
Acetone, 250 ml LD
GHS02GHS07 Danger H225 H319 EUH066 H336
1 6748200  4 
Sea sand, purified, 250 g LD

Articles marked with ** are additionally required.

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