Michealis-Menten kinetics using the enzyme urease

VC6.1.2.2
Michealis-Menten kinetics using the enzyme urease
Michealis-Menten kinetics using the enzyme urease

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Description

In experiment C6.1.2.2 the kinetics of the enzyme urease are examined. The rate of the reaction with different starting concentration is measured. Applying Michaelis-Menten kinetics, the maximal reaction rate vmax and the michaelis constant Km can be determined.

Components of equipment sets

1 524005W2 Mobile-CASSY 2 WiFi LD
1 524220  4  CASSY Lab 2 LD
1 5240671  4  Conductivity adapter S LD
1 529670  4  Conductivity sensor LD
1 607105  8  Magnetic stirrer mini LD
1 30011  4  Saddle base LD
1 30126  4  Stand rod 25 cm, 10 mm Ø LD
1 666543  4  Double, crossed boss head, 0...16 mm LD
1 666555  4  Universal clamp 0...80 mm LD
5 602010  4  Beaker, Boro 3.3, 150 ml, tall LD
5 666851  4  Stirring magnet, 25 mm x 6 mm Ø, circular LD
1 665997  4  Graduated pipette 10 ml LD
1 666003  4  Pipetting ball (Peleus ball) LD
1 665793  4  Volumetric flask, Boro 3.3, 100 ml LD
2 664153  4  Watch glass dish 60 mm Ø LD
2 604170  4  Powder funnel d=65mm LD
1 664043  4  Test tubes, Fiolax, 16 x 160 mm, set of 10 LD
6 667253  4  Rubber stopper solid, 14...18 mm Ø LD
1 667050  4  Test tube rack, plastic, for 9 tubes, 18 mm Ø LD
1 666961  4  Double-ended microspatula, stainless steel, 185 mm LD
1 ADAHCB602H Precision Balance, 600 g /0,01g, with USB interface LD
1 661243  4  Wash bottle PE 500 ml LD
1 6721700  4  Urea, 100 g LD
1 6752810  4  Urease (1 U/mg), 5 g LD
1 6753400  4  Water, pure, 1 l LD
1 additionally required: PC with Windows 7 or higher with WIFI or USB connection

Experiment Instructions

PDF (Experiment description) PDF (Experiment description) C6.1.2.2 Michealis-Menten kinetics using the enzyme urease
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