Separation of a leaf extract with column chromatography

VC2.4.4.1
Separation of a leaf extract with column chromatography
Separation of a leaf extract with column chromatography

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Description

The process of chromatography was developed by Tsvet on leaf pigments at the beginning of the 20th century. Although ridiculed at first, the technique has since become one the most important methods in organic chemistry. In experiment C2.4.4.1, a leaf extract is produced and separated into its components by means of column chromatography.

Components of equipment sets

1 665592  8  Chromatography column, 235 x 20 mm diam. LD
1 665073  8  Dropper funnel, 75 ml, ST 29 LD
2 664101  8  Beaker, DURAN, 100 ml, squat LD
1 602012  8  Beaker, Boro 3.3, 600 ml, tall LD
1 667092  8  Mortar porcelain 70 mm Ø LD
1 667091  8  Pestle 88 mm LD
1 665005 Funnel, Boro 3.3, 100 mm diam. LD
1 661038  8  Round filter, Type 595, 150 mm diam., 100 pcs. LD
1 661161 Narrow-neck glass bottle, amber glass, 100 ml LD
1 602347 Laboratory bottle, 500 ml, GL 45 thread LD
1 665754  8  Measuring cylinder 100 ml, with plastic base LD
1 665756  8  Measuring cylinder 500 ml, with plastic base LD
1 665217 Glass stirring rod, 500 mm x 8 mm diam., set of 10 LD
1 6677977  8  Electronic Balance 440-3N, 200 g : 0.01 g LD
1 665025 Powder funnel, 100 mm diam., plastic LD
1 6610771  8  Warning labels, GHS LD
1 667050  8  Test tube rack, plastic, for 9 tubes, 18 mm Ø LD
1 664043  8  Test tubes, Fiolax, 16 x 160 mm, set of 10 LD
9 667253  8  Rubber stopper solid, 14...18 mm Ø LD
1 666966  8  Spoon-ended spatula, PP, 180 mm LD
1 665953  8  Dropping pipette, 7 x 150 mm, 10 pcs. LD
1 666584  8  Filtration stand for two funnels LD
1 665954  8  Rubber bulbs, 10 pcs LD
1 30002  8  Stand base, V-shaped, small LD
1 30042  8  Stand rod, 47 cm, 12 mm diam. LD
2 30109  8  Bosshead S LD
2 666555  8  Universal clamp 0...80 mm LD
1 6708200  4  Petroleum ether, 90...110 °C, 250 ml LD
1 6721000  4  Glass wool, 10 g LD
1 6748210  4  Sea sand, purified, 1 kg LD
1 6700410  4  Acetone, 1 l LD
1 661058 Silica gel, 35-70 mesh, 500 g LD
1 661082  8  Stopcock grease, 60 g LD
1 additionally required:
green leaves, dry or fresh

Experiment Instructions

PDF (Experiment description) PDF (Experiment description) C2.4.4.1 Separation of a leaf extract with column chromatography
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