Determination of the acidity constant of bromothymol blue

VC4.2.2.1
Determination of the acidity constant of bromothymol blue
Determination of the acidity constant of bromothymol blue Spectra of bromthymol blue solutions at different pH values
Determination of the acidity constant of bromothymol blue

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Description

In experiment C4.2.2.1, the pH-dependent colour of the indicator bromothymol blue is used to determine the pKa value of the dye. The colouration enables the simultaneous determination of the concentration of the protonated (yellow) and non-protonated (blue) form of the indicator, with which, in turn, the Henderson-Hasselbalch equation



can be used to compute the pKa value.

Components of equipment sets

1 467252 Compact spectrometer, complete LD
2 664470 Rectangular cuvette cell, glass, 10 x 10 mm LD
1 664474  4  Rectangular cuvette cell, polystyrene, 10 x 10 mm LD
1 665996  4  Graduated pipette, 5 ml LD
1 666003  4  Pipetting ball (Peleus ball) LD
5 602345  4  Laboratory bottle, 100 ml, GL 45 thread LD
2 665754  4  Measuring cylinder 100 ml, with plastic base LD
2 602782  4  Glass rod, 200 mm, diam. 5 mm LD
1 6677977  8  Electronic Balance 440-3N, 200 g : 0.01 g LD
1 6674781  8  Digital pH-Meter 201 LD
1 6744600  4  Buffer solution set, 250 ml LD
EUH208 EUH210
1 6710800  4  Bromothymol blue solution, 0.1 %, 50 ml LD
1 6746950  4  Hydrochloric acid, 0.1 mol/l, 500 ml LD
GHS05 H290
1 6738410  4  Sodium hydroxide solution, 0.1 mol/l, 500ml LD
GHS05 H290
1 6736710  4  Disodium hydrogen phosphate, 250 g LD
1 6736010  4  Sodium dihydrogenphosphate, 250 g LD
1 664137  8  Beaker, Boro3.3, 100 ml, tall LD
1 6744600  4  Buffer solution set, 250 ml LD
EUH208 EUH210
1 661243  4  Wash bottle PE 500 ml LD
1 6753400  4  Water, pure, 1 l LD
1 additionally required:
PC with Windows XP/Vista/7/8/10 (x86 or x64)

Experiment Instructions

PDF (Experiment description) PDF (Experiment description) C4.2.2.1 Determination of the acidity constant of bromothymol blue
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