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Temperature-dependent enzyme activity using catalase

Temperature-dependent enzyme activity using catalase

LB4.2.3.3

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Description

In this experiment, the temperature dependence of the enzyme catalase is investigated. For this purpose, the splitting of hydrogen peroxide by catalase is carried out at different temperatures and the enzyme activity is measured indirectly via the gas evolution. The students recognise that proteins show low activity at extreme temperatures (cold and hot) and have a temperature optimum.

Components of equipment sets

1	667057 4	Test tube rack metal, 20 mm Ø
1	6045682 4	Powder spatula, steel, 185 mm
1	30945 4	Universal pencil
2	6659541 8	Rubber bulb
2	6659531 4	Dropping pipette 150 mm x 7 mm Ø
1	38221 4	Stirring thermometer -10...+110 °C
3	602022 4	Beaker Boro 3.3, 100 ml, squat
3	6640421 4	Test tube Fiolax 16 mm x 160 mm
1	610010 8	Laboratory safety goggles
1	6753500 4	Hydrogen peroxide, 30 %, 250 ml Danger H302 H318

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Temperature-dependent enzyme activity using catalase

Description

Components of equipment sets

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