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Chromatographic separation of leaf pigments

Chromatographic separation of leaf pigments

VC3.2.3.1

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Description

In experiment V3.2.3.1, leaf pigments are separated out by means of thin layer chromatography. For this purpose, a leaf extract is produced first. That extract is then applied to a TLC film and the TLC is then left to run in a separation chamber. The individual pigments in a leaf can be separated out and identified in this way.

Components of equipment sets

1	664131 4	Beaker Boro 3.3, 400 ml, squat
1	661062 4	Ready-to-use foil: Silica gel, 200 x 200 mm, set of 25
1	665569 4	Microcapillaries
1	667092 4	Mortar 30 ml, 56 mm x 36 mm
1	667091 4	Pestle 88 mm
1	665009 4	Funnel PP 75 mm Ø
1	661034 4	Round filter, Type 595, 110 mm diam., 100 pcs.
1	666966 4	Spoon-ended spatula, PP, 180 mm
2	6659531 4	Dropping pipette 150 mm x 7 mm Ø
2	6659541 8	Rubber bulb
1	666584 4	Filtration stand for two funnels
1	661161 4	Narrow-neck glass bottle, amber glass, 100 ml
1	665752 4	Measuring cylinder 25 ml, with plastic base
1	667027 4	Tweezers, blunt, 130 mm
1	667016 4	Scissors, 200 mm, pointed
2	664157 4	Watch glass dish 125 mm Ø
1	6700410 4	Acetone, 1 l Danger H225 H319 EUH066 H336
1	6742240 4	Petroleum ether, 30...40 °C, 500 ml Danger H224 H304 H315 H336 H411
1	6719500 4	Acetic acid, 99%-100%, 250 ml Danger H226 H314 H290
1	6748210 4	Sea sand, purified, 1 kg

Experiment Instructions

PDF (Experiment description)

C3.2.3.1 Chromatographic separation of leaf pigments (de)

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Chromatographic separation of leaf pigments

Description

Components of equipment sets

Experiment Instructions

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