Recording of a fluorescence spectrum with a spectrometer

VC3.3.1.3
Recording of a fluorescence spectrum with a spectrometer
Recording of a fluorescence spectrum with a spectrometer

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Description

In experiment C3.3.1.3, a solution of the fluorescent dye fluorescein is exposed to the light from a lamp. For this purpose, the spectrometer is arranged at a right angle to the lamp. In this way, the fluorescence spectrum of the dye can be recorded. This can be compared with the absorption spectrum from experiment C3.3.1.2.

Components of equipment sets

2 664470  8  Rectangular cuvette cell, glass, 10 x 10 mm LD
1 467252 Compact spectrometer, complete LD
1 458100  8  Halogen spotlight, 12 V/20 W LD
1 562791  4  Plug-in power supply, 12 V AC LD
1 30002  4  Stand base, V-shaped, small LD
1 30126  4  Stand rod 25 cm, 10 mm Ø LD
1 30111  4  Clamp with jaw clamp LD
1 6045672  4  Double microspatula, steel, 150 mm LD
1 602022  4  Beaker Boro 3.3, 100 ml, squat LD
1 664130  4  Beaker, Boro 3.3, 250 ml, squat LD
1 665754 Measuring cylinder 100 ml, with plastic base LD
1 665996  4  Graduated pipette, 5 ml LD
1 666003 Pipetting ball (Peleus ball) LD
1 665953 Dropping pipette, 7 x 150 mm, 10 pcs. LD
1 665954 Rubber bulbs, 10 pcs LD
1 30942  4  Colouring, red, 10 g LD
1 6720110  4  Fluoresceine, 25 g LD
1 6719740  4  Ethanol, denaturated, 250 ml LD
GHS02GHS07 Danger H225 H319
1 6753400  4  Water, pure, 1 l LD
1 additionally required: PC with Windows 7 or higher with WIFI or USB connection

Experiment Instructions

PDF (Experiment description) PDF (Experiment description) C3.3.1.3 Recording of a fluorescence spectrum with a spectrometer
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