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The Beer-Lambert law

The Beer-Lambert law

VC3.3.2.1

Dilution series of copper tetraammine

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Description

If the extinction of a solution is measured, its concentration can be calculated from the result. That relationship is expressed by the Beer-Lambert law. Along with the concentration c of a solution, the extinction also depends on the thickness d of the layer and on the substance-specific extinction coefficient ε.

In experiment C3.3.2.1, a dilution series is used to study the proportional relationship between extinction and concentration and to determine the extinction coefficient of blue copper tetraammine.

Components of equipment sets

1	524005W 8	Mobile-CASSY 2 WiFi
1	524220 8	CASSY Lab 2
1	524069 8	Immersion photometer S
1	6662605	Holder for immersion photometer S
1	665793	Volumetric flask, Boro 3.3, 100 ml
1	665792 4	Volumetric flask, Boro 3.3, 50 ml
1	664045 4	Test tubes, Fiolax, 30 x 200 mm, set of 10
3	665997 4	Graduated pipette 10 ml
1	666003	Pipetting ball (Peleus ball)
1	667054 4	Test tube rack, for 12 tubes, 32 mm diam.
1	6677977	Electronic Balance 440-3N, 200 g : 0.01 g
1	6729600 4	Copper(II) sulfate-5-hydrate, 100 g Warning H302 H319 H315 H410
1	6703600 4	Ammonia solution, 25 %, 250 ml Danger H314 H335 H400
1	6753400 4	Water, pure, 1 l
1		additionally required: PC with Windows 7 or higher with WIFI or USB connection