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Determination of the acidity constant of bromothymol blue

Determination of the acidity constant of bromothymol blue

VC4.2.2.1

Spectra of bromthymol blue solutions at different pH values

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Description

In experiment C4.2.2.1, the pH-dependent colour of the indicator bromothymol blue is used to determine the pKa value of the dye. The colouration enables the simultaneous determination of the concentration of the protonated (yellow) and non-protonated (blue) form of the indicator, with which, in turn, the Henderson-Hasselbalch equation

can be used to compute the pKa value.

Components of equipment sets

1	467252	Compact spectrometer, complete
2	664470	Rectangular cuvette cell, glass, 10 x 10 mm
1	664474 4	Rectangular cuvette cell, polystyrene, 10 x 10 mm
1	665996 4	Graduated pipette, 5 ml
1	666003 4	Pipetting ball (Peleus ball)
5	602345 4	Laboratory bottle, 100 ml, GL 45 thread
2	665754 4	Measuring cylinder 100 ml, with plastic base
2	602782 4	Glass rod, 200 mm, diam. 5 mm
1	ADAHCB602H	Precision Balance, 600 g /0,01g, with USB interface
1	6674781 8	Digital pH-Meter 201
1	6744600 4	Buffer solution set, 250 ml
1	6710800 4	Bromothymol blue solution, 0.1 %, 50 ml
1	6746950 4	Hydrochloric acid, 0.1 mol/l, 500 ml Warning H290
1	6738410 4	Sodium hydroxide solution, 0.1 mol/l, 500ml Warning H290
1	6736710 4	Disodium hydrogen phosphate, 250 g
1	6736010 4	Sodium dihydrogenphosphate, 250 g
1	664137 8	Beaker, Boro3.3, 100 ml, tall
1	661243 4	Wash bottle PE 500 ml
1	6753400 4	Water, pure, 1 l
1		additionally required: PC with Windows XP/Vista/7/8/10 (x86 or x64)

Experiment Instructions

PDF (Experiment description)

C4.2.2.1 Determination of the acidity constant of bromothymol blue

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Determination of the acidity constant of bromothymol blue

Description

Components of equipment sets

Experiment Instructions

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