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Test for reducing sugar - Fehling’s reaction

Test for reducing sugar - Fehling’s reaction

VC6.1.1.1

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Description

Fehling's solution was developed by Herrmann Fehling in 1848. It is a reaction for the detection of reducing groups, such as aldehyde functions, for example. It makes it possible to differentiate between reducing and non-reducing sugars. Originally it was also used to determine blood sugar content in diabetics by means of titration. Experiment C6.1.1.1 uses the Fehling reagent to analyse glucose, fructose and sucrose.

Components of equipment sets

1	6668475	Magnetic stirrer with heating plate
1	38233 4	Thermometer, -10...+150 °C/1 K
2	665793 4	Volumetric flask, Boro 3.3, 100 ml
1	665212 4	Glass stirring rod 200 x 8 mm Ø
1	602010 4	Beaker, Boro 3.3, 150 ml, tall
1	664132 4	Beaker, Boro3.3, 600 ml, squat
1	665754 4	Measuring cylinder 100 ml, with plastic base
1	ADAHCB602H	Precision Balance, 600 g /0,01g, with USB interface
1	665997 4	Graduated pipette 10 ml
1	666003 4	Pipetting ball (Peleus ball)
1	664045 4	Test tubes, Fiolax, 30 x 200 mm, set of 10
1	667054 8	Test tube rack, for 12 tubes, 32 mm diam.
1	665953 8	Dropping pipette, 7 x 150 mm, 10 pcs.
1	665954 4	Rubber bulbs, 10 pcs
1	666961 4	Double-ended microspatula, stainless steel, 185 mm
1	661243 4	Wash bottle PE 500 ml
1	6721100 4	D(+)-Glucose, 100 g
1	6720700 4	D(-)-Fructose, 50 g
1	6746050 4	D(+)-Sucrose, 100 g
1	6726710 4	Potassium sodium tartrate, 250 g
1	6736800 4	Sodium hydroxide, pellets, 100 g Danger H314 H290
1	6729600 4	Copper(II) sulfate-5-hydrate, 100 g Danger H302 H318 H400 H410
1	6746750 4	Hydrochloric acid, conc., 25 %, 250 ml Danger H314 H335 H290
1	661091 4	Boiling stones 100 g

Experiment Instructions

PDF (Experiment description)

C6.1.1.1 Test for reducing sugar - Fehlings reaction

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Test for reducing sugar - Fehling’s reaction

Description

Components of equipment sets

Experiment Instructions

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