Test for reducing sugar - Fehling’s reaction

VC6.1.1.1
Test for reducing sugar - Fehling’s reaction
Test for reducing sugar - Fehling’s reaction

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Description

Fehling's solution was developed by Herrmann Fehling in 1848. It is a reaction for the detection of reducing groups, such as aldehyde functions, for example. It makes it possible to differentiate between reducing and non-reducing sugars. Originally it was also used to determine blood sugar content in diabetics by means of titration. Experiment C6.1.1.1 uses the Fehling reagent to analyse glucose, fructose and sucrose.

Components of equipment sets

1 6668471  4  Magnetic stirrer with hotplate LD
1 38233  4  Thermometer, -10...+150 °C/1 K LD
2 665793  4  Volumetric flask, Boro 3.3, 100 ml LD
1 665212  4  Glass stirring rod 200 x 8 mm Ø LD
1 602010  4  Beaker, Boro 3.3, 150 ml, tall LD
1 664132  4  Beaker, Boro3.3, 600 ml, squat LD
1 665754  4  Measuring cylinder 100 ml, with plastic base LD
1 6677977  4  Electronic Balance 440-3N, 200 g : 0.01 g LD
1 665997  4  Graduated pipette 10 ml LD
1 666003  4  Pipetting ball (Peleus ball) LD
1 664045  4  Test tubes, Fiolax, 30 x 200 mm, set of 10 LD
1 667054  4  Test tube rack, for 12 tubes, 32 mm diam. LD
1 665953  4  Dropping pipette, 7 x 150 mm, 10 pcs. LD
1 665954  8  Rubber bulbs, 10 pcs LD
1 666961  4  Double-ended microspatula, stainless steel, 185 mm LD
1 661243  4  Wash bottle PE 500 ml LD
1 6721100  4  D(+)-Glucose, 100 g LD
1 6720700  4  D(-)-Fructose, 50 g LD
1 6746050  4  D(+)-Sucrose, 100 g LD
1 6726710  4  Potassium sodium tartrate, 250 g LD
1 6736800  4  Sodium hydroxide, pellets, 100 g LD
GHS05 Danger H314 H290
1 6729600  4  Copper(II) sulfate-5-hydrate, 100 g LD
GHS07GHS09 Warning H302 H319 H315 H410
1 6746750  4  Hydrochloric acid, conc., 25 %, 250 ml LD
GHS05GHS07 Danger H314 H335 H290
1 661091  4  Boiling stones 100 g LD

Experiment Instructions

PDF (Experiment description) PDF (Experiment description) C6.1.1.1 Test for reducing sugar - Fehling’s reaction
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