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The Biuret test

LC5.3.2.1
The Biuret test
The Biuret test

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Description

In this experiment the students get to know a chemical detection for proteins. For this purpose, different samples are mixed with Biuret solution. The students realise that a positive detection is indicated by a violet colouring of the sample. This is caused by a complex formation of the copper ions with the peptide bonds.

Components of equipment sets

1 6726710  4 
Potassium sodium tartrate, 250 g LD
1 6729600  4 
Copper(II) sulfate-5-hydrate, 100 g LD
GHS09GHS05GHS07 Danger H302 H318 H400 H410
1 6738400  4 
Sodium hydroxide solution, diluted, aprox. 2 M, 500 ml LD
GHS05 Danger H314 H290
1 6735700  4 
Sodium chloride, 250 g LD
1 6753410  4 
Water, pure, 5 l LD
1 30945  4 
Universal pencil LD
2 6659531  4 
Dropping pipette 150 mm x 7 mm Ø LD
2 6659541  8 
Rubber bulb LD
1 6045682  4 
Powder spatula, steel, 185 mm LD
1 667057  4 
Test tube rack metal, 20 mm Ø LD
3 6640421  4 
Test tube Fiolax 16 mm x 160 mm LD
3 667253  4 
Rubber stopper solid, 14...18 mm Ø LD
1 610010  8 
Laboratory safety goggles LD
1 667609  4 
Safety gloves, nitrile rubber, size 8 LD
1 6738400  4 
Sodium hydroxide solution, diluted, aprox. 2 M, 500 ml LD
GHS05 Danger H314 H290
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Biology
Technology

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